Enterobacter species can be isolated by plating into MacConkey agar, eosin methylene blue agar or blood agar(6).

Agar could replace advantageously and efficiently animal gelatine.

Diagnosis can also be done by culturing the bacteria on appropriate media such as egg yolk agar-based media or blood agar media Footnote 1Footnote 4.

Once heated, the blood agar becomes brown and chocolate colored, creating chocolate agar that can be used to culture specific species of microorganisms.

Enumeration: Pour plate method using yeast extract dextrose chloramphenicol (CGYE) agar — EN 15789

R: Bacteria are sent on agar slant or lyophilised.

( 21 ) Note that direct plating of aseptically collected tissues on to a minimally selective agar, such as MacConkey agar, is also useful for diagnosis.

The culture medium of the invention is bi-phasic and includes a solid phase containing an egg slope or agar slope; and a liquid phase including a serum and a peptone.

No hemolytic activity was observed on sheep blood agar.

When grown on a nutrient agar containing phenolphthalein diphosphate the bacterium produces phosphatase.

Analytical methods [1]Enumeration: Pour plate method using a yeast extract dextrose chloramphenicol (CGYE) agar — EN 15789.

Method of analysis [1]Enumeration: pour plate method using yeast extract dextrose chloramphenicol (CGYE) agar (EN 15789

spread plate method using bile esculin azide agar (EN 15788

Bottom view of a Sabouraud agar plate with a colony of Trichophyton rubrum var. rodhainii.

They recorded brain activity while the fish slept in an agar solution that immobilized them.