The present invention provides a method for quantifying the levels of allergen-specific human IgG antibody subclasses, particularly of IgG1, IgG2, IgG3 or IgG4 subclasses, using a reverse ELISA immunoenzymatic technique.
In particular a 2-site sandwich-type ELISA wherein chicken polyclonal antibodies and rabbit polyclonal antibodies bind to hepcidin show unprecedented sensitivity.
Preferably, step (c) comprises an ELISA.
A sEPCR ELISA assay is particularly useful for this purpose.
These methods are particularly suitable for the enzyme-linked immunosorbent assay (ELISA).
The immunoassay is preferably an enzyme-linked immunosorbent assay (ELISA).
The invention relates to an immunoenzymatic method for the quantification of protein CETP in plasma, which requires the utilization of fusion protein GST/CETP, the synthetic peptide CETP H486-S496 and polyclonal antibody anti-CEPT H486-S496.
Detection methods include enzyme-linked immunosorbent assay (ELISA) or other immunoassay procedures.
The hydrolase activity of PSMA and PSM' allows the use of an immunoenzymatic assay for their detection.
The present invention provides a method for quantifying the levels of allergen-specific human IgG antibody subclasses, particularly of IgG1, IgG2, IgG3 or IgG4 subclasses, using a reverse ELISA immunoenzymatic technique.
Detection of anti-CaBP-HA antibodies in a mammal is via enzyme linked immunosorbent assay.
A multiplex enzyme-linked immunosorbent assay (ELISA) using chromogenic substrates for detecting multiple analytes is described.
In particular a 2-site sandwich-type ELISA wherein chicken polyclonal antibodies and rabbit polyclonal antibodies bind to hepcidin show unprecedented sensitivity.
By using two monoclonal antibody types as described above, human HO-1 in cells or tissues is quantified by ELISA to thereby detecting stress.
The ELISA IgM test is most commonly used, although there are two types of ELISA tests (IgM and IgG) that can be used.
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