The figure shows the molecular weight transform spectrum obtained from the mass-to-charge spectrum of a 53-base oligonucleotide.

A mass spectrum having a high-accuracy mass axis can be created.

Since the mass and revolving cycle of the ion correspond to each other, a mass spectrum is obtained from a periodic spectrum.

A first mass spectrum and a second mass spectrum of the same ion sample can be analyzed to determine reaction pairs.

Second mass spectral data is then obtained.

Next, a range of mass/charge ratios for each mass value of the mass spectrum data is computed.

The removed protein is subjected to mass spectroscopy to develop a mass spectrum.

Quantification is obtained by normalizing and calibrating obtained mass spectrum against the mass spectrum obtained for an antibody/antigen of known concentration.

Then, the shifted mass spectrum is compared with the first mass spectrum of the ion sample to determine the reaction pairs based on the neutral difference.

A mass spectrum of at least some of the ions is generated using a mass spectrometer.

In some embodiments, the method comprises comparing the mass spectral profile of the sample to the mass spectral profile of a positive or a negative standard.

A mass spectrum of the entire mass range is retrieved for each interval of each peak.

Parent ions from a first sample are passed through the device and parent ion mass spectra and fragmentation ion mass spectra are obtained.

Parent ions from a first sample are passed through the device and parent ion mass spectra and fragmentation ion mass spectra are obtained.

Thereafter, a set of known masses are identified from the mass spectrum data by associating each peak intensity value in the spectrum with its molecular mass.