All tests must include a known C. m. subsp.sepedonicus control.

In C. m. sepedonicus the value is usually around 5 %.

recognising Serbia as being free from Clavibacter michiganensis ssp. sepedonicus (Spieckerman and Kotthoff) Davis et al.

Serbia is recognised as being free from Clavibacter michiganensis ssp. sepedonicus (Spieckerman and Kotthoff) Davis et al.

Use validated C. m. subsp. sepedonicus-specific oligo-probes (Appendix 7).

The pathogenicity test must be performed as final confirmation of a diagnosis of C. m. subsp. sepedonicus and for assessment of virulence of cultures identified as C. m. subsp. sepedonicus

recognising Bosnia and Herzegovina as being free from Clavibacter michiganensis ssp. sepedonicus (Spieckerman and Kotthoff) Davis et al.

The bioassay test is positive if the test plants are infected by C. m. subsp. sepedonicus.

(a) The tubers are originating from countries which are known to be free of Clavibacter michiganensis spp. sepedonicus (Spieckermann and Kotthoff) Davis et al.

Also a Gram stain can be prepared: this test will differentiate C. m. subsp. sepedonicus from Erwinia spp.

aliquots of resuspended pellets to which C. m. subsp. sepedonicus has been added (preparation see Appendix 2

Detection of Clavibacter michiganensis ssp. sepedonicus in samples of potato tubers and plants

Scheme for detection and identification of Clavibacter michiganensis ssp. sepedonicus in samples of asymptomatic potato plants

For detection of latent C. m. subsp. sepedonicus populations it is advised to test composite samples.

It is hereby recognised that Bulgaria is recognised as being free from Clavibacter michiganensis (Smith) Davis et al. ssp. sepedonicus (Spieckerman and Kotthoff) Davis et al.