A single 3' termination sequence, such as a polyadenylation sequence when the construct is to be expressed in eucaryotic cells, follows the last coding sequence.

Bicistronic constructs contain a single 5' promoter, protein encoding sequence 1, an IRES, protein encoding sequence 2, and a single 3' polyadenylation sequence.

The vectors transduce a complete transcriptional unit containing a promoter, a protein coding sequence and a polyadenylation sequence into the genome of mammalian cells.

For instance, the vector may include, from upstream to downstream, a first promoter, at least one cloning site, a rat Kv2.1 polyadenylation sequence, and an origin of replication.