If necessary, the culture water may be renewed by pouring or siphoning the used water from culture vessels carefully without destroying the tubes of larvae.

concentrations tested: the nominal test concentrations and any results of analyses to determine the concentration of the test substance in the test vessels.

C. riparius and C. yoshimatsui emergence to adults from control vessels should occur between 12 and 23 days after their insertion into the vessels; for C. tentans, a period of 20 to 65 days is necessary.

Appropriate amounts of formulated sediment (see paragraphs 13-14 and Appendix 3) are added in the test vessels to form a layer of at least 1,5 cm.

Aeration of the water has to be stopped while adding the larvae to test vessels and remain so for another 24 hours after addition of larvae (see paragraphs 25 and 32).

Aeration of the water has to be stopped while adding the larvae to test vessels and remain so for another 24 hours after addition of larvae (see paragraphs 24 and 32).

The Bunsen burner flame can be used to help maintain a sterile field around the openings of experimental containers.

A randomised design for location of the test vessels in the incubator is required to minimise the influence of spatial differences in light intensity or temperature.

Other techniques to introduce the test substance into the test vessels may be used as described in (7) and (8).

concentrations tested: the nominal test concentrations and any results of analyses to determine the concentration of the test substance in the test vessels.

Test vessels and other apparatus, which will come into contact with the test solutions should be made entirely of glass or other chemically inert material.

concentrations tested: the nominal test concentrations and any results of analyses to determine the concentration of the test substance in the test vessels.

He swapped experimental containers in the Biolab Incubator containing bacteria cultures that could be used for carbon dioxide removal and oxygen production.