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Template-directed ligation of tethered mononucleotides by t4 dna ligase for kinase ribozyme selection.... ATP concentrations above 33 microM accumulated adenylated intermediate and decreased yields of the gap-sealed product, likely due to re-adenylation of dissociated enzyme....
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Template-directed ligation of tethered mononucleotides by t4 dna ligase for kinase ribozyme selection... ATP concentrations above 33 mM accumulated adenylated intermediate and decreased yields of the gap-sealed product, likely due to re-adenylation of dissociated enzyme....
Template-directed ligation of tethered mononucleotides by t4 dna ligase for kinase ribozyme selection... ATP concentrations above 33 µM accumulated adenylated intermediate and decreased yields of the gap-sealed product, likely due to re-adenylation of dissociated enzyme....

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