The invention provides a method for screening, isolation and recovery of clones using self-ligation of inverse PCR products.
By comparison with other techniques used to genotype inversions one by one, like inverse PCR, iMLPA has shown a very high sensibility, reproducibility and accuracy.
Another aspect of the invention includes the reverse amplification of circular indicator by using center sequence of indicator as reverse PCR primers.
The reverse PCR of the circular indicator DNA by using center sequence of indicator as reverse primers will replace the direct test PCR.
In one embodiment, the algae includes a DNA construct having polymerase chain reaction forward primer (302), a inducible promoter (304), a PSII-iRNA sequence (306), a terminator (308), and a PCR reverse primer (310).
The methods include reverse PCR and site-directed mutagenesis for generating chimera and truncations or site-directed mutations of enzymes, respectively.
If reverse PCR analysis is available, then its results can be used in the choice of treatment options.
Requêtes fréquentes français :1-200, -1k, -2k, -3k, -4k, -5k, -7k, -10k, -20k, -40k, -100k, -200k, -500k, -1000k,
Requêtes fréquentes anglais :1-200, -1k, -2k, -3k, -4k, -5k, -7k, -10k, -20k, -40k, -100k, -200k, -500k, -1000k,
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