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The d-alanyl-d-alanine-cleaving peptidases and β-lactamases. structure and mechanismpeer reviewedEnzyme kinetics, amino acid and nucleotide sequencing, oligonucleotide-directed mutagenesis, protein crystallography and X-ray diffraction, computer molecular graphics and theoretical chemistry contribute much to the unravelling of...
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Rapid and reliable site-directed mutagenesis using kunkel’s approach... Common among them is the oligonucleotide-directed mutagenesis (1)....
Oligonucleotide-directed mutagenesis as a general and powerful method for studies of protein functionWe have used oligonucleotide-directed mutagenesis to make a specific change in the ß -lactamase (EC 3.5.2.6)...
Oligonucleotide-directed mutagenesis as a general and powerful method for studies of protein function.We have used oligonucleotide-directed mutagenesis to make a specific change in the beta-lactamase (EC 3.5.2.6)...
A simple vector modification to facilitate oligonucleotide-directed mutagenesis.We describe a simple modification of commonly used single-stranded cloning vectors that permits the efficient recovery of mutant DNA molecules in oligonucleotide-directed mutagenesis experiments, even when the absolute efficiency of mutagenesis is very low....

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