The sample is extracted with acidified methanol.

After addition of an internal standard, the sample is extracted with acidified methanol.

Add 100,0 ml of acidified methanol (3.8) by pipette.

Make up to the mark with acidified methanol (3.8) and mix.

Dilute to the mark with acidified methanol (3.8) and mix thoroughly.

Add 100,0 ml of acidified methanol (3.8) and swirl to disperse.

Diclazuril is eluted from the cartridge with a mixture of acidified methanol and water.