The present invention relates to methods for increasing the level of zeaxanthin in a plant line.

The male sterile parental plant line is maintained by physically separating the progeny seeds by colour sorting.

The method can also be reversed, which means that a plant cell is transformed with a construct encoding a nucleic acid sequence to be excised, and the crossing is performed witha plant line containing a DSBI.

The ability of the trait to be expressed in the modified plant line and plant lines derived there from in a consistent, reliable, and predictable manner.

The ability of the transgenic trait to be expressed in the transformed plant line and plant lines derived therefrom in a consistent, reliable, and predictable manner.