Cell cultures are treated with a spindle inhibitor (e.g. colchicine) one to four hours prior to harvesting.
Prior to euthanasia, animals are treated with a metaphase-arresting agent (e.g. colchicine or colcemid).
The assay consists in adding cytochalasin-B (an inhibitor of the mitotic spindle that prevents cytokinesis) to cell cultures.
identity of metaphase arresting substance, its concentration and duration of treatment
Requêtes fréquentes français :1-200, -1k, -2k, -3k, -4k, -5k, -7k, -10k, -20k, -40k, -100k, -200k, -500k, -1000k,
Requêtes fréquentes anglais :1-200, -1k, -2k, -3k, -4k, -5k, -7k, -10k, -20k, -40k, -100k, -200k, -500k, -1000k,
Traduction Translation Traducción Übersetzung Tradução Traduzione Traducere Vertaling Tłumaczenie Mετάφραση Oversættelse Översättning Käännös Aistriúchán Traduzzjoni Prevajanje Vertimas Tõlge Preklad Fordítás Tulkojumi Превод Překlad Prijevod 翻訳 번역 翻译 Перевод