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gène indicateur

reporter gene

sciences naturelles et appliquées / santé - acta.es iate.europa.eu

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Microrna-138 regulates hypoxia-induced endothelial cell dysfunction by targeting s100a1... Transfection of a MiR-138 mimic into ECs reduced S100A1–3 ‘UTR reporter gene expression, while transfection of an anti MiR-138 prevented the hypoxia-induced downregulation of the reporter gene....

santé - core.ac.uk - PDF: ftp:

Noninvasive visualization of microrna-16 in the chemoresistance of gastric cancer using a dual reporter gene imaging system... Here, we introduced a dual reporter gene imaging system for noninvasively monitoring the kinetic expression of miRNA-16 during chemoresistance in gastric cancer both in vitro and in vivo....

PDF: figshare.com

Noninvasive visualization of microrna-16 in the chemoresistance of gastric cancer using a dual reporter gene imaging system.... Here, we introduced a dual reporter gene imaging system for noninvasively monitoring the kinetic expression of miRNA-16 during chemoresistance in gastric cancer both in vitro and in vivo....

PDF: doaj.org

Reporter gene expression levels in transgenic mice and non-transgenic littermate.... (D) Reporter gene expression levels in various organs from transgenic mice....

PDF: figshare.com

Optimization of biguanide derivatives as selective antitumor agents blocking adaptive stress responses in the tumor microenvironment... We conducted luciferase reporter assays using stable cell lines expressing either an HIF-1-responsive reporter gene or a glucose-regulated protein 78 promoter-reporter gene, which were induced by hypoxia and glucose deprivation stress, respectively, to screen for TME-targeting...

PDF: doaj.org

gène indicateur

indicator gene

santé - iate.europa.eu

La présente invention concerne un exemple d'un tel gène indicateur.

The present invention provides an example of such an indicator gene.

sciences naturelles et appliquées - wipo.int

Si un fragment de gène avec une structure de lecture ouverte est inséré dans un vecteur, le gène indicateur peut être réactivé.

If a gene fragment with an open reading frame is inserted into a vector, the indicator gene may be reactivated.

sciences naturelles et appliquées - wipo.int

On identifie une protéine servant de modulateur de la transcription du gène recherché en détectant un signal généré par le gène indicateur.

A protein is identified as a modulator of the transcription of the gene of interest by detecting a signal generated by the indicator gene.

sciences naturelles et appliquées - wipo.int

L'expression d'un gène effecteur est mesurée par une réaction RT-PCR quantitative et compétitive, et l'abondance d'un gène indicateur est mesurée par une réaction PCR quantitative et compétitive.

Effector gene expression is measured by competitive quantitative RT-PCR, and indicator gene abundance is measured by competitive quantitative PCR.

politique de l'environnement - wipo.int

Dans certains modes de réalisation, la cellule hôte comporte un segment dérivé d'un patient et un gène indicateur.

In certain embodiments, the host cell comprises a patient- derived segment and an indicator gene.

sciences naturelles et appliquées - wipo.int

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Neutralization of diverse hiv-1 isolates by polyclonal antibodies in plasma of hiv-1-infected subjects from thailand and north america.... as the reciprocal of the plasma dilution that produced a 50% inhibition in relative light units (RLU) in U87 target cells infected with pseudoviruses expressing a luciferase indicator gene under transcriptional control of the HIV-1 Tat gene....

général - core.ac.uk - PDF: figshare.com

High-frequency intracellular transposition of a defective mammalian provirus detected by an in situ colorimetric assay.We devised an indicator gene for retrotransposition, nlsLacZRT, which contains the Escherichia coli lacZ gene fused to a nuclear location signal (nlsLacZ), engineered in such a way that the...

PDF: www.pubmedcentral.nih.gov

Retrotransposition of a marked drosophila line-like i element in cells in culture.We have marked a Drosophila transposable element--the LINE-like I element--with an intron-containing indicator gene inserted in place of a large deletion in the I element second ORF encompassing the reverse transcriptase domain, and this marked element was placed downstream to...

PDF: www.pubmedcentral.nih.gov

The hormone response element of the mouse mammary tumour virus dna mediates the progestin and androgen induction of transcription in the proviral long terminal repeat region.... Chimaeric constructs containing the HRE and the authentic LTR promoter linked to an indicator gene or the HRE linked to an otherwise hormone insensitive promoter directing the transcription of an indicator gene, were transfected into the human mammary tumour ...

PDF: www.pubmedcentral.nih.gov

A tetrahymena thermophila ribozyme-based indicator gene to detect transposition of marked retroelements in mammalian cellsWe devised an indicator gene for retrotransposition based on an autocatalytic ribozyme element—the Tetrahymena thermophila 23S rRNA group I intron—which can self-splice in vitro and does not...

PDF: www.pubmedcentral.nih.gov