A cloned gene encoding protein G, or functionally active portions thereof, vectors containing the cloned gene, and microorganisms transformed by those vectors.
The invention also concerns the use of a cloned gene in the Gramineae to identify and isolate the orthologous gene sequence in apomictic plants.
The cloned gene can produce proteins utilizable as a medicine or the like.
The method is not limited by a required induction of the cloned gene by addition of exogenous inducers.
Further, a transformed plant having bluer petals than usual has been obtained by introducing the cloned gene into a plant and expressing the same.
1974 First expression in bacteria of a gene cloned from a different species.
1977: The first expression of a human gene in bacteria.
The invention also provides for a replicating viral vector capable of expressing a cloned exogenous gene, wherein the vector demonstrates reduced virulence due to at least one mutation in the coding sequence of a structural protein of a virus.
A lysine-rich protein gene has been cloned from winged bean, Psophocarpus tetragonolobus and its DNA sequence and deduced protein sequence are disclosed.
For example, antibodies generated against the expression product of a gene cloned from genomic or cDNA libraries can be used to characterize the product and, hence, illuminate the function of the gene.
The present invention presents a Kunitz-type recombinant inhibitor obtained from a gene cloned from a cDNA library of salivary glands of the Amblyomma cajennense, and the inhibitor named Amblyomin-X has molecular mass of 13.500 Da.
The invention specifically relates to transfection methods that increase levels of expression of a cloned gene product and eukaryotic cell lines derived from these methods.
A cloned gene or fragment thereof encodes antigenic proteins that bind with a monoclonal or polyvalent antibody that is directed against an antigenic protein of avian coccidia.
A process for making DNA libraries in filamentous fungal cells using a novel cloned gene involved in the mismatch repair system of filamentous fungal cells.
A cloned gene or fragment thereof encodes antigenic proteins that bind with a monoclonal or polyvalent antibody that is directed against an antigenic protein of avian coccidia.
The present invention also relates to the cloning and expression of the wild type or mutant Tne DNA polymerase in E. coli, to DNA molecules containing the cloned gene, and to host cells which express said genes.
The present invention also relates to the cloning and expression of the wild type or mutant DNA polymerases in E. coli, to DNA molecules containing the cloned gene, and to host cells which express said genes.
Some viral vaccines (hepatitis-B, etc.) consist of purified antigenic proteins manufactured after expression from a gene cloned into a suitable vector ( e.g., yeast).
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