The choice of conical tube must be adapted to the pestle.

The choice of conical tube must be adapted to the pestle.

Decant the liquid through the weighed filter crucible, retaining the fibres in the flask.

The vacuum is released to prevent the column from drying and a conical flask is placed under the cartridge.

Fold the filtration membrane in four minimum and put it in the 15 ml conical tube.

The filtration membrane is folded (minimal) in four and put in the 15 ml conical tube.

The filtration membrane is folded (minimal) in four and put in the 15 ml conical tube.

Follow the procedure described in the general instructions and proceed as follows: to the specimen contained in the conical flask of at least 200 ml capacity, add 100 ml of formic acid per gram of specimen.

To the specimen contained in the glass-stoppered conical flask of at least 200 ml capacity, add 100 ml glacial acetic acid per gram of specimen.

To the specimen contained in the glass-stoppered conical flask of at least 200 ml capacity, add 100 ml of the azeotropic mixture per gram of specimen.

Reflux condenser (suitable for liquids of high boiling point), fitting the conical flask (a).

10 to 20 ml of water are put into the dropping funnel of the apparatus and 10 g of substance are put in the conical flask.

To the specimen contained in the conical flask, add 100 ml of benzyl alcohol per gram of specimen.

To the specimen contained in the conical flask (3.1(a)), add 100 ml of xylene (3.2) per gram of specimen.

Transfer soluble lysate to a conical flask and add 2 mL of resin (see Table of Materials).