Ms Sansbury noted that when Cas9 cuts DNA, the result can be “blunt ends,” while Cpf1 produces “sticky ends.

Sansbury noted that when Cas9 cuts DNA, the result can be "blunt ends," while Cpf1 produces "sticky ends.

Sansbury noted that when Cas9 cuts DNA, the result can be “blunt ends,” while Cpf1 produces “sticky ends.

Cpf1, on the other hand, leaves “sticky ends” that make it easier for a researcher to insert new DNA.

Using a linker-primer and an adaptor, cDNA is prepared to have two different sticky ends which can be ligated to those of the vector.

Such ends are called sticky or cohesive ends because base pairing between them can stick the DNA molecule together again.