The oxidoreductase may be, for example, a laccase, peroxidase, or diaphorase.
The enzymes (13, 14) are, for example, diaphorase and glucose dehydrogenase.
Disclosed is a protein that provides a mutant diaphorase that has improved residual diaphorase activity over the wild type even after a certain amount of heat treatment.
DT-diaphorase is the prerequisite two-electron reducing enzyme which carries out the activation process.
The selectivity is believed to relate to differential substrate specificity for DT-diaphorase.
The invention relates to a kit for measuring the amount of analyte in a sample, comprising a tablet comprising a tetrazolium salt, diaphorase and NAD+, and at least one enzyme active on the analyte to be measured is described.
The mass ratio between the poly-L-lysine and glutaraldehyde in the immobilization material is set at from 5:1 to 80:1, and the mass ratio between glucose dehydrogenase and diaphorase is set at from 1:3 to 200:1.
[ii] Bhatnagar M, Sukhwal P, Suhalka P, Jain A, Joshi C, Sharma D. Effects of fluoride in drinking water on NADPH-diaphorase neurons in the forebrain of mice: A possible mechanism of fluoride neurotoxicity.
[ii] Bhatnagar M, Sukhwal P, Suhalka P, Jain A, Joshi C, Sharma D. Effects of fluoride in drinking water on NADPH-diaphorase neurons in the forebrain of mice: A possible mechanism of fluoride neurotoxicity.
[ii] [24] Bhatnagar M, Sukhwal P, Suhalka P, Jain A, Joshi C, Sharma D. Effects of fluoride in drinking water on NADPH-diaphorase neurons in the forebrain of mice: A possible mechanism of fluoride neurotoxicity.
[ii] [24] Bhatnagar M, Sukhwal P, Suhalka P, Jain A, Joshi C, Sharma D. Effects of fluoride in drinking water on NADPH-diaphorase neurons in the forebrain of mice: A possible mechanism of fluoride neurotoxicity.
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