An enriched population of regulatory cells can be obtained by harvesting the non-adherent cells in the culture medium of a monolayer culture of PO chondrocytes.

These 32K proteins specifically inhibit basal secretion of FSH, but not of LH, in a rat anterior pituitary monolayer culture system.

2008 ・Study on an eye irritation evaluation method based on a combination of SIRC-CVS test and the three-dimensional cultured skin model test

In accordance with the guidelines, the cells in suspension or monolayer culture should be exposed to the test substance, with and without metabolic activation, for a range of 3–6 hours.

These proteins specifically inhibit basal secretion of FSH, but not of LH, in a rat anterior pituitary monolayer culture system.

Cells in suspension or monolayer culture are exposed to the test substance, both with and without metabolic activation, for a defined period of time.

Cells in suspension or monolayer culture are exposed to, at least four analysable concentrations of the test substance, both with and without metabolic activation, for a suitable period of time.

This 34.5kD protein specifically inhibits basal secretion of FSH, but not of LH, in a rat anterior pituitary monolayer culture system, exhibiting a half-maximal effective dose of 0.3 ng/ml.

Cells in suspension or monolayer culture are exposed to, at least four analysable concentrations of the test substance, both with and without metabolic activation, for a suitable period of time.

The 28 kD protein promotes secretion of FSH, but not of LH, in a rat anterior pituitary monolayer culture system, exhibiting an EC50 of 0.5 ng/ml.

The tests involve a cell culture for reproducing the virus and three tests to determine viral foci on a cell overlay, the macrophage infection rate and the production of IFN-alpha.

Cells in suspension or monolayer culture are exposed to, at least four analysable concentrations of the test substance, both with and without metabolic activation, for a suitable period of time.