The inclusion bodies may then be purified and the protein of interest may be isolated after cleavage from the inclusion body tag.
The inclusion bodies may then be purified and the protein of interest may be isolated after cleavage from the inclusion body tag.
The present invention relates to an isolated inclusion body comprising a polypeptide, characterised in that such inclusion body is in particulate form.
The present invention also refers to a bacterial cell comprising said inclusion body.
Inclusion Body Myopathy Associated with Paget's Disease of Bone and Frontotemporal Dementia
The inclusion bodies may be of solid, liquid or gaseous nature and may be mechanically robust.
Such Fc receptors can easily be obtained by expressing respective nucleic acids in prokaryotic host cells and renaturation of the obtained inclusion bodies, which procedure leads to a very homogenous and pure product.
Ultrastructurally, abnormal inclusion bodies are observed in fibroblasts, bone marrow histiocytes and lymphocytes.
Boas with inclusion body disease often have a history of vomiting, disinterest in food, weight loss and skin problems.
This invention provides a process for obtaining bioactive recombinant protein from inclusion bodies without unfolding it completely into random coil structure.
The inclusion bodies may be collected and purified easily by altering the ionic strength and/or pH of media used to dissolve the inclusion bodies.
The inclusion bodies are isolated and are solubilized under denaturing conditions.
The proteins in the inclusion bodies are in an insoluble and inactive form.
The proteins in the inclusion bodies are in an insoluble and inactive form.
It might be that the inclusion bodies are only found by coincidence in sick boas and that there is in fact no connection between the illness and the inclusion bodies.
The gene product was purified from inclusion bodies.
New methods for the production of recombinant polypeptides from inclusion bodies are disclosed.
The second method of the present invention is to enhance the production of target proteins in the cytoplasm and also convert the target proteins from soluble form to insoluble inclusion body, using ibpA and/or ibpB gene-amplified bacteria.
The present invention moreover refers to a composition comprising said inclusion body and animal or plant tissue.
The present invention additionally refers to a composition comprising said inclusion body and a eukaryotic cell.
In a similar manner cell wall particles may be separated from a protein inclusion body suspension.
These inclusion bodies formed by therapeutic proteins could be used for the treatment of different diseases.
The method employs a polypeptide recognizing Chlamydia infected inclusion body components.
The invention further comprises washing and solubilising of the inclusion bodies, especially under non-denaturating conditions.
The inclusion bodies are used to generate binding partners which bind specifically to said (poly)peptides.
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