Collect the distillate in a 100 ml volumetric flask and make up to the mark with water.

Weigh 55,4 mg PEDP (4.1) into a 50 ml volumetric flask (5.6) and add about 25 ml of chloroform (4.2.2) using a graduated cylinder (5.11).

Pipette 1 ml (5.3) of this solution into a 10 ml volumetric flask (5.6) and make up to the mark with methanol (4.2.1) in order to obtain a 0,15 mM tryptamine concentration

Instead of directly measuring dimensions or using glassware like a volumetric flask, it is necessary to make use of a liquid displacement method to measure the volume of an irregularly shaped solid.

Volume of graduated flask in ml

After cooling, transfer quantitatively to a graduated flask (point 5.1.1) and make up to the mark with water.