Annealing (French : annelage) Hybridization of a synthetic oligonucleotide to a single strand nucleic acid.
Methods for quantitating amplified DNA and determining its purity are carried out by analysis of melting and reannealing curves.
Annealing (French : annelage) Hybridization of a synthetic oligonucleotide to a single strand nucleic acid.
The transcription-free SELEX method assembles nucleic acid ligands from fragments of synthetic nucleic acids by annealing those fragments to a complementary template, and then ligating the fragments together.
In one embodiment, the genes, about 500 to 4000 base pairs long, are made by annealing and extending overlapping 60mer oligonucleotides followed by cloning into a plasmid vector.
The primer molecules anneal to the denatured DNA template and the DAN polymerase catalyzes elongation of the primer.
In one preferred embodiment, a method of elongating a primer annealed to a DNA template is disclosed.
In a second step a methylation specific oligonucleotide carrying a non-extendable 3’ end is annealed to the converted DNA.
Amplification products, varying in length depending on the transposon insertion site, are obtained using one primer that anneals to the target sequence and a second primer that anneals to the transposon.
A first oligonucleotide having a 3' amino group and a second oligonucleotide having a 5' phosphate group are annealed to a contiguous complementary region of a target nucleic acid.
In a single reaction, the primer is annealed to the target DNA and extended in an extension/polymerization reaction to produce copies of the target DNA comprising the primer.
Requêtes fréquentes français :1-200, -1k, -2k, -3k, -4k, -5k, -7k, -10k, -20k, -40k, -100k, -200k, -500k, -1000k,
Requêtes fréquentes anglais :1-200, -1k, -2k, -3k, -4k, -5k, -7k, -10k, -20k, -40k, -100k, -200k, -500k, -1000k,
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