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sciences naturelles et appliquées - iate.europa.eu
Cloning regions of the drosophila genome by microdissection of polytene chromosome dna and pcr with nonspecific primer.... The procedure involves microdissection of the region of interest, amplification of the DNA by PCR using a primer designed to prime the synthesis nonspecifically, labeling of the amplified DNA using the random primer method, and screening of a standard library with the probe to identify and isolate clones carrying sequences homologous to the dissected region....
général - core.ac.uk - PDF: www.pubmedcentral.nih.gov

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sciences naturelles et appliquées - acta.es


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