The ligases comprise fusion proteins including a DNA ligase and a DNA binding protein, e.g., a T4 DNA ligase with an N-terminal p50 fusion.
In this step, a specific enzyme called DNA ligase is used.
Finally, a third enzyme called DNA ligase joins the new molecules back together.
A DNA ligase is an enzyme which can repair DNA by creating new covalent bonds between two pieces ofDNA.
Ligation of the cleavage products with a DNA ligase corrects non-variant cleavages and thus substantially reduces background.
After gel purifying the digests, the insert is ligated or joined to the plasmid, via an enzyme called DNA ligase.
Adaptors (short, double-stranded pieces of synthetic DNA) are then ligated to these fragments with the help of DNA ligase, an enzyme that joins DNA strands.
The present invention discloses a novel polypeptide, a human DNA ligase 21, the polynucleotide encoding the polypeptide and the mothod for producing the polypeptide by DNA recombinant technology.
A DNA ligase is an enzyme which can repair DNA by creating new covalent bonds between two pieces ofDNA.
The present invention discloses a novel polypeptide -human DNA ligase 9 and a polynucleotide encoding the same, as well as a method of producing the polypeptide by DNA recombinant technique.
Once inserted into a plasmid, the EPO gene can be glued in place using another enzyme called DNA ligase.
The present invention discloses a novel polypeptide, DNA ligase 10, the polynucleotide encoding the polypeptide and the method for producing the polypeptide by DNA recombinant technology.
The present invention discloses a novel polypeptide, a human DNA ligase 15, the polynucleotide encoding the polypeptide and the method for producing the polypeptide by DNA recombinant technology.
This involves the use of a reaction mixture that includes a DNA ligase and an effective amount of at least one endonuclease as well as a cofactor selected from NAD+ or ATP.
The invention relates to methods using a modified DNA ligase which increases the discrimination of perfect matches from mismatches for complementary polynucleotides.
Next, scientists bond the source DNA to the vector with a DNA ligase enzyme that repairs the splice and creates a single DNA strand.
This invention provides methods for the discovery of molecules that target an essential step of bacterial DNA replication - the sealing of DNA strands by NAD+-dependent DNA ligase.
A yeast homologue of mammalian DNA ligase IV is provided and a role for DNA ligase IV established in the Ku-associated DNA repair pathway.
a chemical that stimulates a cell to commence cell division, triggering mitosis (i.e. cell division).
The invention provides isolated maize DNA Ligase II nucleic acids and their encoded proteins.
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