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Tyrosine hydroxylase activity and extrinsic fluorescence changes produced by polyanions.... We used the apolar fluorescent probe 8-anilino-1-naphthalenesulphonic acid (1,8-ANS) as a reporter group to provide the first evidence for changes in conformation related to changes in activity....
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Chloride-dependent uncoupling mediated by oligomycin in rat liver mitochondria...phosphorylation, stimulation of adenosine triphosphatase activity, release of the respiratory control, decrease of energy-dependent changes in the fluorescence of the dye 8-anilino-1-naphthalenesulphonic acid and rapid swelling of mitochondria....
Binding of a burst-phase intermediate formed in the folding of denatured d-glyceraldehyde-3-phosphate dehydrogenase by chaperonin 60 and 8-anilino-1-naphthalenesulphonic acid.... The fully unfolded enzyme, however, is re-activated only to a limited extent after dilution, and refolds rapidly in a burst phase to a partially folded intermediate characterized by increases in both the emission intensity of intrinsic fluorescence and binding to 8-anilino-1-naphthalenesulphonic acid (ANS)....
The compact state of reduced bovine pancreatic trypsin inhibitor is not the compact molten globule ... Optical rotatory dispersion in the visible region failed to show the presence of pronounced secondary structures in the reduced UPTI and no binding or 8-anilino-1-naphthalenesulphonic acid to reduced BPTI could be detected....
Alternative ligands as probes for the carotenoid-binding site of lobster carapace crustacyanin.The apoproteins of the lobster carotenoprotein, crustacyanin, show single high-affinity binding sites for the hydrophobic fluorescence probes 8-anilo-1-naphthalenesulphonic acid and cis-parinaric acid, and exhibit fluorescence transfer from tryptophan to the ligands....

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