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The −11a of promoter dna and two conserved amino acids in the melting region of σ70 both directly affect the rate limiting step in formation of the stable rna polymerase-promoter complex, but they do not necessarily interact... The likely nucleation site is the highly conserved −11A base in the non-template strand of the −10 promoter region....
général - core.ac.uk - PDF: www.pubmedcentral.nih.gov
Processing of topoisomerase i cleavable complexes into dna damage by transcription.... By contrast, CPT-stabilized TOP1 cleavable complexes located on the non-template strand within the transcribed region was unaffected by the elongating RNA polymerase....
Region 1.2 of the rna polymerase σ subunit controls recognition of the −10 promoter element... σ also functions as an elongation factor, inducing transcription pausing by interacting with transcribed DNA non-template strand sequences that are similar to the −10 element sequence....

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