Assuming that rice globulin accumulated in vacuole-origin protein granules II would have a vacuole migration signal, it is attempted to identify the vacuole migration signal.

Said selective inhibitor has been found to inhibit vacuolar H+-ATPase, such as vacuolar H+-ATPase in osteoclast cells, whereby it is therapeutically efficient against physiological disorders involving bone resorption.

The present invention provides compositions and methods for regulating vacuolar pH.

Vacuolar ATPase inhibitors are selected among products of natural, semi-synthetic or synthetic origin.

The use of vacuolar ATPase inhibitors in the prevention and/or treatment of septic shock is herein described.

The activity of inhibitors of vacuolar-type H+-ATPases in the inhibition of vacuolation induced by Helicobacter pylori is disclosed.

The invention is accomplished in part by silencing the vacuolar acid invertase gene using RNAi technology.

The method of the present invention includes administering a vacuolar-type (H+)-ATPase inhibiting-effective amount of a compound of the present invention.

The first covering layer has a thickness of at least 4 µm and the base layer contains vacuoles.

The inhibitor may be a primary amine, a carboxylic ionophore, or a selective inhibitor of the vacuolar proton pump (V-ATPase).

The first cover layer has a thickness of at least 4 µm and the base layer is vacuolar.

The invention also is directed toward a vacuolar localization sequence.

The invention is further directed to a method for purifying and using the substrate-specific aminopeptidase.

The method involves targeting the enzyme to the vacuoles through the use of a barley polyamine oxidase (BPAO) vacuole sorting signal peptide.

In particular, DNA constructs encoding tomato plant vacuolar invertase in operative linkage with a developmentally regulated promoter region are provided.