The template DNA is removed and the first extension product is primed and copied by the reverse primer to produce a second extension product.

The second extension product then dissociates from the first extension product and thus from the substrate and re-hybridizes to another first primer molecule that has not extended.

The second primer then hybridizes to and extends based on the first extension product, at a location 3' of the complement of the nucleotide of interest, so as to incorporate the nucleotide of interest in a second extension product.

The labeled extension product is detected by hybridization of the 5' portion to the preselected sequence.

The invention further relates to prevention of replication of a primer extension product in PCR reactions.

The non-extended first primer then extends from a location 3' of the nucleotide of interest in the second extension product, so as to form, in combination with the second extension product, a double-stranded nucleic acid fragment.

A primer extension product is generated with fixed a length determined by the length of the ECS.

(3) Expansion port 3 Connects the computer to an optional docking device or expansion product.

(3) Expansion port 3 Connects the computer to an optional expansion product or docking device.

In another embodiment, the detection method includes an in situ primer extension step that allows the production of the extension product within an intact cell.

Methods and compositions are provided for detecting a primer extension product in a reaction mixture.

Target regions of single-stranded template DNA are primed and copied by the forward primer to produce a first extension product.

You can also install GROHE Sense+ if you want both the extension and a mains powered product.

You can also install GROHE Sense+ if you want both the extension and a mains powered product.

In this embodiment, the extension product can be preserved under appropriate conditions for an extended time before the completion of the quantification step.