Plating and Pre-incubation of Cells for Testing

Tubes should be aerated during pre-incubation by using a shaker.

The test can also be performed without pre-incubation at 30 °C.

The measurement procedure can also be performed without pre-incubation at 30 oC.

The procedures described in the method pertain primarily to the plate incorporation and preincubation methods.

The measurement procedure can also be performed without pre-incubation at 30 oC.

It is also recognised that certain classes of mutagens are not always detected using standard procedures such as the plate incorporation method or preincubation method.

The inoculation may be preceded by pre-incubation of the dilutions with the antiserum to IPN virus at appropriate dilution as described in point I.4.3.

Preincubation is carried out by adding insect cell membrane expressing human P glycoprotein to a reaction buffer solution containing vanadic acid and [3H] ATP.

The inhibitory activity for the P fimbriated bacteria was nondialyzable and was detected only after preincubation of the bacteria with the cocktail.

Subjecting cultured neurons to estrogen pre-incubation also prevented glutamate-mediated neurotoxicity, a common mechanism of neuronal death.

Intracellular inhibition of virus replication was also noted on pre-incubation of uninfected cells in medium containing cactus extract with removal of the extract prior to virus infection of cells.