The recombinant DNA of the present invention may further comprise a polyadenylation signal sequence.
A 3' untranslated region may be provided having a polyadenylation signal.
There is disclosed an expression vector utilizing an internal polyadenylation signal.
A polyadenylation nucleotide sequence from soybean is further shown.
Preferably, at least one putative yeast polyadenylation consensus sequence in said nucleic acid has been modified.
One polyadenilation signal is identified at position 951.
polyA_site site on an RNA transcript to which will be added adenine residues by post-transcriptional polyadenylation
polyA_signal recognition region necessary for endonuclease cleavage of an RNA transcript that is followed by polyadenylation; consensus=AATAAA
These microarrays preferably represent alternate polyadenylation sequences that are specific to the tissue from which the transcripts are derived.
The improvement involves using a reporter cassette containing a DNA sequence encoding a cytochrome P450 with a polyadenylation signal sequence as the reporter gene.
A single 3' termination sequence, such as a polyadenylation sequence when the construct is to be expressed in eucaryotic cells, follows the last coding sequence.
The novel polynucleotides and expression vectors may comprise an immunoglobulin gene polyadenylation signal from an IgG 1 heavy-chain gene.
The DNA sequence corresponds to a retroviral genome that is reverse transcriptase and/or integrase and where the 3'LTR has been replaced by a heterologous polyadenylation sequence.
Optionally the artificial nucleic acid molecule may further comprise, e.g. a poly(A)sequence, a poyladenylation signal, and/or a 3'UTR.
The invention relates to a modified retrotransposable element wherein one or more polyadenylation sites have been removed from within the genes of the retrotransposable element, and methods of use thereof.
These mRNAs are stably expressed, lack polyadenylation tails, and allow minimal protein translation except when in the presence of specific proteins.
In one embodiment, the constructs contain two or more enzyme-encoding genes, each under the control of an inducible promoter and each with a polyadenylation signal.
The present invention shows that this optimally succinct sequence has similar activity to the SV40 polyadenylation signal that is currently used in expression vectors.
The method makes use of polyadenylation, primers, and general probes for the detection of RNA sequences including mRNAs and short RNA sequences such as miRNA.
The above comprises an expressed construct, with a sequence coding for an immunomodulatory protein or polypeptide under the control of a functional promoter and a functional polyadenylation signal.
Requêtes fréquentes français :1-200, -1k, -2k, -3k, -4k, -5k, -7k, -10k, -20k, -40k, -100k, -200k, -500k, -1000k,
Requêtes fréquentes anglais :1-200, -1k, -2k, -3k, -4k, -5k, -7k, -10k, -20k, -40k, -100k, -200k, -500k, -1000k,
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