The invention relates to antibodies and antigen-binding fragments thereof which bind to proadrenomedullin N-terminal peptide ("PAMP").

The glycated protein may be glycated hemoglobin, which is preferably treated with a protease to cleave out a β-chain N-terminal amino acid or β-chain N-terminal peptide therefrom.

Disclosed is a method for cleaving an amide bond in a peptide chain, thereby obtaining an N-terminal peptide as a free amine and a secondary residue, as well as a peptide obtained by such method.

Blood-based fibrosis biomarkers such as procollagen type III N-terminal peptide (PIIINP) can be useful in the non-invasive detection or monitoring of a fibrotic process.