The enzymes immobilized by the above process include Penicillin acylase from rE.coli RE III (pKA18), Novel Penicillin acylase from Achromobacter sp (CCM4834) expressed in rE.coli BL21 (pK1P1) CCM 7394 and rE.coli RE III(pKX1P1).
The invention consists in a nucleotide sequence having the size of (2646) bp, wherein the order of nucleotides is identical to the order of the nucleotide sequence encoding penicillin acylase from Achromobacter sp.
The sequence can be used in the formation of a DNA construct, eventually the construct having at least one regulatory sequence regulating the expression of the gene and the production of a polypeptide with the penicillin acylase activity.
On the other hand, the novel blend of grafted biopolymers immobilized industrial enzymes such as lipases, glucose oxidase, penicillin acylase, and others and it showed a synergistic effect in terms of enzyme's loading capacity.
The invention relates to mutated penicillin acylases having a high synthesis/hydrolysis ratio in comparison with wild-type penicillin acylases and at least 1% of the activity.
Requêtes fréquentes français :1-200, -1k, -2k, -3k, -4k, -5k, -7k, -10k, -20k, -40k, -100k, -200k, -500k, -1000k,
Requêtes fréquentes anglais :1-200, -1k, -2k, -3k, -4k, -5k, -7k, -10k, -20k, -40k, -100k, -200k, -500k, -1000k,
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