Furthermore, a device for isoelectrical focussing, comprising a so-called IEF chamber (IsoElectrical Focussing chamber) and a power supply is also disclosed, whereby the power supply delivers voltages of = 10 kV.
The system still uses current IEF systems for first-dimensional separation.
IEF run in the device of the invention gives high protein resolution and enables analysis of low abundant proteins.
A protective sheath for use with an immobilized pH gradient (IPG) strip in an isoelectric focusing (IEF) process is generally disclosed.
A device for isoelectric focusing.
A device and method for isoelectric focusing of ampholytes in a buffer uses a cone-shaped capillary with a positive electrode connected at a narrow end and a negative electrode connected at the wide end of the capillary.
These substances are useful as carrier ampholytes in isoelectric focusing.
These muteins have higher homogeneity when subjected to isoelectric focusing.
Also disclosed is a digital isoelectric focusing method using the devices and methods.
The method entails using capillary electrophoresis or capillary isoelectric focusing to separate intact microbes.
This is determined by isoelectric focusing or other appropriate techniques.
Novel ampholyte analysis process entails analyzing chemical compounds, usually ampholytes, used in isoelectric focusing processes.
Novel ampholyte analysis process entails analyzing chemical compounds, usually ampholytes, used in isoelectric focusing processes.
Some commonly used electrophoretic methods are: SDS-PAGE, native-PAGE and isoelectric focusing.
The present invention relates to the field of isoelectric focussing or 2D electrophoresis, in particular isoelectric point markers with fluorescence detection.
In the first dimension, proteins are separated based on their isoelectric point (pI).
In the first dimension, proteins are separated according to their isoelectric point (pI).
This invention provides a device for separation of mixtures of especially biological molecules into components by isoelectric focussing.
The poly(NAT + CL) gels were found to be advantageous also for isoelectric focussing in carrier ampholytes or immobilized pH gradients.
The use of more than 10,000 volts over a distance of more than 30 cm for the isoelectrical focussing is also disclosed.
Additionally, proteins can be isolated according their charge using electrofocusing.[43]
Additionally, proteins can be isolated according to their charge using electrofocusing.[44]
Additionally, proteins can be isolated according to their charge using electrofocusing.[44]
Additionally, proteins can be isolated according their charge using electrofocusing.[39]
Additionally, proteins can be isolated according their charge using electrofocusing.[42]
Additionally, proteins can be isolated according their charge using electrofocusing.[43]
Additionally, proteins can be isolated according their charge using electrofocusing.[42]
Each trough is shaped to retain an elongate strip of isoelectric focusing medium and sufficient liquid to immerse the elongate strip.
In many embodiments, the invention provides methods and apparatus that permit isoelectric focusing in the absence of oil-based or hydrophobic cover layers.
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