a K270 extinction coefficient not exceeding 0,90

Nearest-neighbor extinction coefficients at 260 nm:

a K270 extinction coefficient not higher than 0,25

a K270 extinction coefficient not higher than 0,25

a K270 extinction coefficient not exceeding 0,90

The per cent quench shall be calculated as follows

thickness(D), reflective index(N) and extinction coefficient(K).

and recorded as Hm The per cent water quench shall be calculated as follows

This method uses the A280 absorbance value in combination with either the mass extinction coefficient or the molar extinction coefficient to calculate the concentration of the purified protein.

The indolenine compound is represented by the following general formula (1).

water quench according to paragraph 9.3.9.2.2.: 3 per cent of full scale

quench according to paragraph 9.3.9.2.1.: 2 per cent of full scale

The extinction coefficient of a protein can be given by online servers19.

If the extinction coefficient is unknown, measure the absorbance of the additive at different concentrations, plot the absorbance vs. concentration and calculate the extinction coefficient from the slope of the curve.

If the concentration of the solute is expressed in moles per liter, the constant ε is called the molar extinction coefficient.