Open Reading Frame: Open reading frame (ORF) refers to the sequence of DNA or RNA that is located between the initiation codon (start-code sequence) and termination codon (stop-code sequence).

It consists of 2379 nucleotides, the initiation codon TTG of this gene is from nucleotides 201 to 203 in its 5' end, and the termination codon TGA of this gene is from nucleotides 2577 to 2579 in its 5' end.

Some SNPs cause amino acid substitutions, while others introduce a chain termination codon, rendering a truncated product.

Said nucleotide mutation is located at the second exon of the GS3 gene, in which a cysteine codon (TGC) in the small-grain group is mutated to a termination codon (TGA) in the large-grain group.

The method relies on the introduction of a termination codon into the coding sequence of the toxic protein and complementation with a suppressor tRNA.

By allowing for a pause at a terminating stop codon and addition of release factor the final proteon may be obtained in almost pure form.

The previously published hPMS2 cDNA sequence lacks an upstream in-frame stop codon preceding the presumptive initiating methionine.

This cycle is repeated until a stop codon is encountered on an mRNA.

3'UTR region at the 3? end of a mature transcript (following the stop codon) that is not translated into a protein

3'UTR region at the 3' end of a mature transcript (following the stop codon) that is not translated into a protein