A method of identifying transcriptional terminator sequences or attenuator sequences is also provided.

A nonsense mutation in DNA results in a premature stop codon within an mRNA.

Some people are homozygotes for a genetic variant of FUT2 which is characterised by an enzyme-inactivating nonsense change at position 143 of the amino acid sequence, so that a tryptophan codon is converted into a stop codon.

And a mutation to for a stop code-on is called a nonsense mutation.

The methods result in the stimulation or inhibition of a change in reading frame or stop codon readthrough during translation.

And a mutation to for a stop code-on is called a nonsense mutation.

The gene used does not include sequences from regions at i 5' of the first translated ATG and ii 3' of the stop codon of the EPO gene.

Said regulator gene contains a stop codon situated before the translation starting site and flanked by loxP elements, in order to ensure the Cre-dependent expression thereof.

This premature stop codon in the mRNA causes disease by terminating translation before a full-length protein is generated.

The programming effort requires an algorithm that initiates lead sequences and generates the variable regions until an end codon is encountered.

Pyrrolysine and selenocysteine are encoded via variant codons; for example, selenocysteine is encoded by stop codon and SECIS element.

PCR primers are designed and used to link the α-galactosidase gene to the cellobiohydrolase promoter and terminator so that the α-galactosidase is expressed and expelled from the organism.

The invention particularly relates to using any gene encoding a premature stop codon to identify compounds that modulate premature translation termination and/or nonsense-mediated mRNA decay.

Ataluren enables ribosomal readthrough of mRNA containing such a premature stop codon, resulting in production of a full-length protein.

A method of identifying latent splice sites, the method comprising identifying at least one intronic in-frame stop codon located upstream of a 5' splice site sequence being a latent splice site, thereby identifying latent splice sites.